A bacterial global regulator forms a prion

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Science  13 Jan 2017:
Vol. 355, Issue 6321, pp. 198-201
DOI: 10.1126/science.aai7776

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Prions enter another domain of life

Prions are self-propagating protein aggregates, discovered in connection with the fatal transmissible spongiform encephalopathies in mammals. Prions have also been identified in fungi, where they act as protein-based elements of inheritance. Although prions have been uncovered in evolutionarily diverse eukaryotic species, it is not known whether prions exist in bacteria. Yuan and Hochschild report the identification of a bacterial protein—the transcription termination factor Rho from Clostridium botulinum—that exhibits the defining hallmarks of a prion-forming protein.

Science, this issue p. 198


Prions are self-propagating protein aggregates that act as protein-based elements of inheritance in fungi. Although prevalent in eukaryotes, prions have not been identified in bacteria. Here we found that a bacterial protein, transcription terminator Rho of Clostridium botulinum (Cb-Rho), could form a prion. We identified a candidate prion-forming domain (cPrD) in Cb-Rho and showed that it conferred amyloidogenicity on Cb-Rho and could functionally replace the PrD of a yeast prion-forming protein. Furthermore, its cPrD enabled Cb-Rho to access alternative conformations in Escherichia coli—a soluble form that terminated transcription efficiently and an aggregated, self-propagating prion form that was functionally compromised. The prion form caused genome-wide changes in the transcriptome. Thus, Cb-Rho functions as a protein-based element of inheritance in bacteria, suggesting that the emergence of prions predates the evolutionary split between eukaryotes and bacteria.

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