Nucleic acid detection with CRISPR-Cas13a/C2c2

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Science  28 Apr 2017:
Vol. 356, Issue 6336, pp. 438-442
DOI: 10.1126/science.aam9321

Sensitive and specific CRISPR diagnostics

Methods are needed that can easily detect nucleic acids that signal the presence of pathogens, even at very low levels. Gootenberg et al. combined the allele-specific sensing ability of CRISPR-Cas13a with recombinase polymerase amplification methods to detect specific RNA and DNA sequences. The method successfully detected attomolar levels of Zika virus, as well as the presence of pathogenic bacteria. It could also be used to perform human genotyping from cell-free DNA.

Science, this issue p. 438