Reports
Nucleic acid detection with CRISPR-Cas13a/C2c2
- Jonathan S. Gootenberg1,2,3,4,5,*,
- Omar O. Abudayyeh1,2,3,4,6,*,
- Jeong Wook Lee7,
- Patrick Essletzbichler1,2,3,4,
- Aaron J. Dy1,4,8,
- Julia Joung1,2,3,4,
- Vanessa Verdine1,2,3,4,
- Nina Donghia7,
- Nichole M. Daringer8,
- Catherine A. Freije1,9,
- Cameron Myhrvold1,9,
- Roby P. Bhattacharyya1,
- Jonathan Livny1,
- Aviv Regev1,10,
- Eugene V. Koonin11,
- Deborah T. Hung1,
- Pardis C. Sabeti1,9,12,13,
- James J. Collins1,4,6,7,8,†,
- Feng Zhang1,2,3,4,†
- 1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
- 2McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.
- 3Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
- 4Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
- 5Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
- 6Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
- 7Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
- 8Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
- 9Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.
- 10Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
- 11National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
- 12Department of Immunology and Infectious Disease, Harvard School of Public Health, Boston, MA 02115, USA.
- 13Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.
- ↵†Corresponding author. Email: zhang{at}broadinstitute.org (F.Z.); jimjc{at}mit.edu (J.J.C.)
↵* These authors contributed equally to this work.
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Science 28 Apr 2017:
Vol. 356, Issue 6336, pp. 438-442
DOI: 10.1126/science.aam9321
Vol. 356, Issue 6336, pp. 438-442
DOI: 10.1126/science.aam9321
Jonathan S. Gootenberg
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
Omar O. Abudayyeh
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Jeong Wook Lee
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
Patrick Essletzbichler
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Aaron J. Dy
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Julia Joung
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Vanessa Verdine
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Nina Donghia
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
Nichole M. Daringer
Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Catherine A. Freije
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.
Cameron Myhrvold
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.
Roby P. Bhattacharyya
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Jonathan Livny
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Aviv Regev
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Eugene V. Koonin
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
Deborah T. Hung
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Pardis C. Sabeti
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.Department of Immunology and Infectious Disease, Harvard School of Public Health, Boston, MA 02115, USA.Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.
James J. Collins
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Feng Zhang
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
