Reports
Nucleic acid detection with CRISPR-Cas13a/C2c2
- View ORCID ProfileJonathan S. Gootenberg1,2,3,4,5,*,
- View ORCID ProfileOmar O. Abudayyeh1,2,3,4,6,*,
- View ORCID ProfileJeong Wook Lee7,
- View ORCID ProfilePatrick Essletzbichler1,2,3,4,
- View ORCID ProfileAaron J. Dy1,4,8,
- Julia Joung1,2,3,4,
- View ORCID ProfileVanessa Verdine1,2,3,4,
- Nina Donghia7,
- View ORCID ProfileNichole M. Daringer8,
- View ORCID ProfileCatherine A. Freije1,9,
- View ORCID ProfileCameron Myhrvold1,9,
- View ORCID ProfileRoby P. Bhattacharyya1,
- View ORCID ProfileJonathan Livny1,
- View ORCID ProfileAviv Regev1,10,
- View ORCID ProfileEugene V. Koonin11,
- View ORCID ProfileDeborah T. Hung1,
- View ORCID ProfilePardis C. Sabeti1,9,12,13,
- View ORCID ProfileJames J. Collins1,4,6,7,8,†,
- View ORCID ProfileFeng Zhang1,2,3,4,†
- 1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
- 2McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.
- 3Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
- 4Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
- 5Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
- 6Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
- 7Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
- 8Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
- 9Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.
- 10Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
- 11National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
- 12Department of Immunology and Infectious Disease, Harvard School of Public Health, Boston, MA 02115, USA.
- 13Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.
- ↵†Corresponding author. Email: zhang{at}broadinstitute.org (F.Z.); jimjc{at}mit.edu (J.J.C.)
↵* These authors contributed equally to this work.
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Science 28 Apr 2017:
Vol. 356, Issue 6336, pp. 438-442
DOI: 10.1126/science.aam9321
Vol. 356, Issue 6336, pp. 438-442
DOI: 10.1126/science.aam9321
Jonathan S. Gootenberg
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
2McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.
3Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
4Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
5Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
Omar O. Abudayyeh
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
2McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.
3Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
4Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
6Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Jeong Wook Lee
7Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
Patrick Essletzbichler
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
2McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.
3Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
4Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Aaron J. Dy
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
4Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
8Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Julia Joung
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
2McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.
3Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
4Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Vanessa Verdine
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
2McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.
3Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
4Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Nina Donghia
7Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
Nichole M. Daringer
8Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Catherine A. Freije
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
9Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.
Cameron Myhrvold
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
9Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.
Roby P. Bhattacharyya
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Jonathan Livny
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Aviv Regev
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
10Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Eugene V. Koonin
11National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
Deborah T. Hung
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Pardis C. Sabeti
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
9Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.
12Department of Immunology and Infectious Disease, Harvard School of Public Health, Boston, MA 02115, USA.
13Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.
James J. Collins
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
4Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
6Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
7Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
8Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Feng Zhang
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
2McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.
3Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
4Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
