A selfish genetic element confers non-Mendelian inheritance in rice

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Science  08 Jun 2018:
Vol. 360, Issue 6393, pp. 1130-1132
DOI: 10.1126/science.aar4279
  • Fig. 1 Identification of qHMS7.

    (A to D) Morphology (upper panels) and stained pollen (lower panels) of DJY1, Mer, hybrid F1 (Mer × DJY1), and NIL-qHMS7. Fertile pollen is stained dark and sterile pollen is not stained. Scale bars: 50 cm in the upper panels and 50 μm in the lower panels. (E) Pollen fertility of DJY1, Mer, hybrid F1, and NIL-qHMS7, shown as mean ± SD (n = 5, 5, 7, and 10 plants examined, respectively). **P < 0.01 (versus DJY1, by Student’s t test). (F) ORFs predicted in the fine-mapped region. The insertion of ORF3 in DJY1 is indicated with a gray triangle. Arrows indicate the orientation of ORFs.

  • Fig. 2 ORF3 performs a pollen-protection function in a gametophytic manner.

    (A) A schematic of expected genotypes of the gametes from the ORF3 T0 transgenic plants (genotype D/M, ORF3/-) and the resulting T1 progeny from selfing. T1 plants of the (M/M; ORF3/-) and (M/M; ORF3/ORF3) genotypes are highlighted in green ovals. (B) Genotype and pollen fertility of the T1 plants from a selfed T0 transgenic plant (genotype D/M; ORF3/-; line Q040). Scale bars, 50 μm. The observed and expected plant numbers of the corresponding genotype among 162 T1 progeny are shown by N and (NE), respectively. Pollen fertility of the T1 plants of various genotypes is shown as mean ± SD. The genotype and pollen fertility of two additional T1 families are shown in table S4.

  • Fig. 3 The evolutionary origin and a genetic action model of ORF2 and ORF3.

    (A) The ancestral wild rice has an ancestral genotype of ORF2, but lacks ORF3. On the basis of haplotype combinations of ORF2 and ORF3, AA-genome wild rice and cultivated rice can be grouped into four types (I to IV). Their frequencies are shown under each type. ORF2M, ORF2N, and ORF2D are shown in gray, purple, and wine red, respectively, and ORF3 is shown in green. (B) A diagram shows that in the hybrids of type IV O. rufipogon and Asian rice (genotype: 2D/2D; 3D/3D) with other ancestral AA-genome wild rice or cultivated rice (genotypes 2M/2M; -/- or 2N/2N; -/-), pollen of the genotype 2M; - or 2N; - is aborted owing to the lack of protective ORF3.

Supplementary Materials

  • A selfish genetic element confers non-Mendelian inheritance in rice

    Xiaowen Yu, Zhigang Zhao, Xiaoming Zheng, Jiawu Zhou, Weiyi Kong, Peiran Wang, Wenting Bai, Hai Zheng, Huan Zhang, Jing Li, Jiafan Liu, Qiming Wang, Long Zhang, Kai Liu, Yang Yu, Xiuping Guo, Jiulin Wang, Qibing Lin, Fuqing Wu, Yulong Ren, Shanshan Zhu, Xin Zhang, Zhijun Cheng, Cailin Lei, Shijia Liu, Xi Liu, Yunlu Tian, Ling Jiang, Song Ge, Chuanyin Wu, Dayun Tao, Haiyang Wang, Jianmin Wan

    Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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    • Materials and Methods 
    • Figs. S1 to S20
    • Tables S1 to S9
    • References 

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