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Science  31 Aug 2018:
Vol. 361, Issue 6405, pp. 929
DOI: 10.1126/science.361.6405.929

Glass Media Bottles

PUREGRIP borosilicate glass media bottles use a cap with a protruding ridge that improves a user's ability to safely handle the bottle with gloved hands, especially when the bottles are wet. The patented cap is easy to open or close, and provides a wide, flat surface for writing and labeling. Constructed of durable 3.3 low-expansion ASTM E438 Type I Class A borosilicate glass, PUREGRIP bottles come in sizes of 100 mL, 250 mL, 500 mL, 1 L, 2 L, 3 L, 5 L, 10 L, and 20 L. Each bottle in the product line includes volumetric gradations that have been individually certified for accuracy.

Contained Filtration System

The SupaClean system from Amazon Filters is designed for filtration applications where quick cleandowns are required in a multipurpose production site, or where hazardous materials are being processed and risk of operator exposure needs to be minimized. The system consists of Amazon Filter's standard range of high-performance filter cartridges enclosed inside two sealed plastic bags, all of which are contained inside a stainless-steel housing that provides the pressure vessel required for the filtration process. SupaClean is ideally suited to the production of coatings. Extensive validation testing ensures that the plastic bags enclosing the filters and product always remain attached even when full of liquid. Filter changeout is quick and simple—the filter assembly can be simply lifted out of the housing and replaced by a new unit, with minimal risk of the operator or surrounding area encountering the product. 

Secondary Antibodies 

Brilliant Violet 421 and Brilliant Violet 480 conjugated secondary antibodies allow you to add more colors to your multiple labeling assays in the violet–blue region of the spectrum. When combined with Alexa Fluor 488, Rhodamine Red-X, and Alexa Fluor 647 conjugates, effective five-color fluorescent labeling is possible. If nuclear counterstaining is desired, four-color antibody staining is possible using Brilliant Violet 421, Brilliant Violet 480, Alexa Fluor 488, and Rhodamine Red-X. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5 (which has red emission) frees the violet–blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5's excitation and emission profiles overlap those of Alexa Fluor 647. We offer a range of secondary antibodies that are recommended for multiple labeling due to their minimal cross-reactivity.

Chemically Competent and Electrocompetent Cells

Vmax Express is a novel, fast-growing bacterial strain designed and optimized for high-level recombinant protein expression. This rationally engineered, next-generation prokaryotic protein expression system can serve as a replacement for slow-growing Escherichia coli systems that are prone to low yields and the expression of proteins as insoluble inclusion bodies. Vmax cells are derived from the marine microorganism Vibrio natriegens. This gram-negative, nonpathogenic bacterium has a doubling time twice that of E. coli and produces three to four times its biomass. Utilizing a robust transcription and translation system to support this rapid growth rate, Vmax Express generates more biomass and more recombinant protein per liter faster. It is compatible with plasmids and antibiotics commonly used with bacterial expression systems such as E. coli BL21(DE3). Designed with a tightly controlled, IPTG-inducible T7 promoter system, Vmax Express cells can be cultured using routine growth medium as well as commercial autoinduction media, or our Vmax Enriched Growth Media.

Lentivector Plasmid and Prepackaged Virus

System Biosciences' family of Lenti-Labeler constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient, reliable labeling of your cells. The pLL-CMV-GFP-T2A-Puro Lenti-Labeler construct expresses copepod green fluorescent protein (GFP) from the cytomegalovirus (CMV) promoter, which delivers strong expression in most commonly used cell lines (HeLa, HEK293, HT1080, etc.), and coexpresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA or ready-to-transduce prepackaged lentivirus, pLL-CMV-GFP-T2A-Puro Lenti-Labeler is designed for reliability, so you can get valuable insights faster.

F-Actin Recombinant Protein

Achieve fast staining and immediate functional analysis of filamentous actin (F-actin) in living and fixed cells with ibidi's new reagent, LifeAct-TagGFP2 Protein. The addition of this recombinant protein extends a nontoxic, noninvasive alternative to phalloidin. F-actin is a key component of the cytoskeleton and is involved in many important cellular processes, such as cell division, cell migration, and endocytosis. LifeAct—a 17-amino-acid peptide derived from a protein found in yeast—specifically binds F-actin structures in living or fixed eukaryotic cells, while uniquely retaining full actin dynamics. Conjugated with green fluorescent protein (GFP), it can be conveniently introduced into cells, making it the ideal tool for F-actin labeling. With LifeAct-TagGFP2 Protein, scientists can easily transfect living cells, then get brilliant fluorescence microscopy images of cellular F-actin structures for analysis within minutes. 

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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