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Comment on “Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water”

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Science  31 Aug 2018:
Vol. 361, Issue 6405, eaar7101
DOI: 10.1126/science.aar7101


Riback et al. (Reports, 13 October 2017, p. 238) used small-angle x-ray scattering (SAXS) experiments to infer a degree of compaction for unfolded proteins in water versus chemical denaturant that is highly consistent with the results from Förster resonance energy transfer (FRET) experiments. There is thus no “contradiction” between the two methods, nor evidence to support their claim that commonly used FRET fluorophores cause protein compaction.

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