Research Article

Developmental barcoding of whole mouse via homing CRISPR

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Science  31 Aug 2018:
Vol. 361, Issue 6405, eaat9804
DOI: 10.1126/science.aat9804

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Lineage tracing in mouse using CRISPR

A homing guide RNA (hgRNA) that directs CRISPR-Cas9 to its own DNA locus can diversify its sequence and act as an expressed genetic barcode. Kalhor et al. engineered a mouse line carrying 60 independent loci of hgRNAs, thus generating a large number of unique barcodes in various embryonic and extraembryonic tissues in fully developed mice. This method demonstrates lineage tracing from the very first branches of the development tree up to organogenesis events and was used to elucidate embryonic brain patterning.

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