The Ly6/uPAR protein Bouncer is necessary and sufficient for species-specific fertilization

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Science  07 Sep 2018:
Vol. 361, Issue 6406, pp. 1029-1033
DOI: 10.1126/science.aat7113

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Bouncer keeps fertilization specific

Fertilization needs to be highly efficient while remaining species-specific. However, despite decades of research, it is still unclear how these two requirements are met. Herberg et al. report the discovery of the Ly6/uPAR-type protein Bouncer as a species-specific fertilization factor in zebrafish (see the Perspective by Lehmann). Bouncer localizes to the egg membrane and is required for sperm entry. Remarkably, expression of Bouncer from another fish species (medaka) in zebrafish allowed for cross-species fertilization.

Science, this issue p. 1029; see also p. 974


Fertilization is fundamental for sexual reproduction, yet its molecular mechanisms are poorly understood. We found that an oocyte-expressed Ly6/uPAR protein, which we call Bouncer, is a crucial fertilization factor in zebrafish. Membrane-bound Bouncer mediates sperm-egg binding and is thus essential for sperm entry into the egg. Remarkably, Bouncer not only is required for sperm-egg interaction but is also sufficient to allow cross-species fertilization between zebrafish and medaka, two fish species that diverged more than 200 million years ago. Our study thus identifies Bouncer as a key determinant of species-specific fertilization in fish. Bouncer’s closest homolog in tetrapods, SPACA4, is restricted to the male germline in internally fertilizing vertebrates, which suggests that our findings in fish have relevance to human biology.

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