Joint profiling of chromatin accessibility and gene expression in thousands of single cells

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Science  28 Sep 2018:
Vol. 361, Issue 6409, pp. 1380-1385
DOI: 10.1126/science.aau0730

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Single-cell chromatin and RNA analysis

Single-cell analyses have begun to provide insight into the differences among and within the individual cells that make up a tissue or organism. However, technological barriers owing to the small amount of material present in each single cell have prevented parallel analyses. Cao et al. present sci-CAR, a pooled barcode method that jointly analyzes both the RNA transcripts and chromatin profiles of single cells. By applying sci-CAR to lung adenocarcinoma cells and mouse kidney tissue, the authors demonstrate precision in assessing expression and genome accessibility at a genome-wide scale. The approach provides an improvement over bulk analysis, which can be confounded by differing cellular subgroups.

Science, this issue p. 1380