Research Article

Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells

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Science  26 Oct 2018:
Vol. 362, Issue 6413, eaau1783
DOI: 10.1126/science.aau1783

Imaging chromatin spatial organization

The genome is organized within the nucleus as three-dimensional domains that modulate DNA-templated processes. Bintu et al. used high-throughput Oligopaint labeling and imaging to observe chromatin dynamics inside the nuclei of several different mammalian cell lines. After combining the datasets, single-cell matrices revealed chromatin arranged in topologically associating domains (TADs). Removing cohesin resulted in a loss of aggregate TADs among populations of cells, but specific TADs were still detected at the single-cell level. Furthermore, higher-order organization was detected, suggestive of cooperative interactions within the genome.

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