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Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry

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Science  16 Nov 2018:
Vol. 362, Issue 6416, pp. 829-834
DOI: 10.1126/science.aau0976

Article Information

vol. 362 no. 6416 829-834

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History: 
  • Received for publication May 6, 2018
  • Accepted for publication October 8, 2018

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Author Information

  1. Dror S. Chorev1,
  2. Lindsay A. Baker2,
  3. Di Wu1,
  4. Victoria Beilsten-Edmands1,
  5. Sarah L. Rouse3,
  6. Tzviya Zeev-Ben-Mordehai2,*,
  7. Chimari Jiko4,
  8. Firdaus Samsudin5,
  9. Christoph Gerle6,7,
  10. Syma Khalid5,
  11. Alastair G. Stewart8,9,
  12. Stephen J. Matthews3,
  13. Kay Grünewald2,10,
  14. Carol V. Robinson1,
  1. 1Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QZ, UK.
  2. 2Division of Structural Biology, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.
  3. 3Department of Life Sciences, Imperial College, London, South Kensington Campus, London SW7 2AZ, UK.
  4. 4Institute for Integrated Radiation and Nuclear Science, Kyoto University, Kumatori, Japan.
  5. 5School of Chemistry, University of Southampton, University Road, Southampton SO17 1BJ, UK.
  6. 6Institute for Protein Research, Osaka University, Suita, Osaka, Japan.
  7. 7Core Research for Evolutional Science and Technology, Japan and Science and Technology Agency, Kawaguchi, Japan.
  8. 8Molecular, Structural and Computational Biology Division, Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia.
  9. 9Faculty of Medicine, The University of New South Wales, Sydney, NSW, Australia.
  10. 10Centre of Structural Systems Biology (CSSB), Notkestr. 85, D-22607, Heinrich-Pette Institute/University of Hamburg, Hamburg, Germany.
  1. Corresponding author. Email: carol.robinson{at}chem.ox.ac.uk
  • * Present address: Cryo–Electron Microscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht 3584 CH, Netherlands.

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