Long-term functional maintenance of primary human hepatocytes in vitro

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Science  26 Apr 2019:
Vol. 364, Issue 6438, pp. 399-402
DOI: 10.1126/science.aau7307

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Keep hepatocytes fresh

Freshly isolated primary human hepatocytes (PHHs) quickly lose their identity and function in vitro, limiting their application in modeling infectious liver diseases and screening drugs. Xiang et al. describe a simple and effective five-chemical culture condition that can maintain the mature function of cultured PHHs for an extended period of time. PHHs cultured in this way can support the entire viral life cycle and recapitulate long-term infection of the hepatitis B virus. This system created a useful drug-screening platform for developing new antiviral strategies.

Science, this issue p. 399