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Science  07 Jun 2019:
Vol. 364, Issue 6444, pp. 996
DOI: 10.1126/science.364.6444.996

Light-Sheet Microscope

Combining Olympus' BX43 upright frame and optics with PhaseView's advanced multiview selective plane illumination technology, the Alpha³ is a cost-effective light microscopy solution. The Alpha³ light-sheet microscope system provides high temporal and spatial resolution of both fixed and live biological samples for 3D imaging. The optional Smart 3D scanning technology allows for fast z-stacking and perturbation-free acquisition at 75 images/s. In addition to increased speed, an important benefit of light sheet microscopy is reduced phototoxicity. The system's multiview selective plane illumination in combination with real-time laser focus sweeping permits ultrathin optical sectioning for illumination homogeneity, increased axial resolution, and enhanced signal-to-noise response compared to traditional confocal techniques. The modularity of the Alpha³ system's components, including a full selection of detection objectives (2X–60X), extends the benefits of light-sheet microscopy to a wider range of academic research fields. The system can be adapted to fit specific experimental needs and budgets.

Automated Confocal Microscope

ZEISS Celldiscoverer 7 is a fully integrated, high-end imaging system with various incubation and detection options. It combines the convenient automation of a boxed microscope with the image quality and flexibility of a classic inverted research microscope. To get better data from 3D samples, it is now possible to add ZEISS LSM 900 with Airyscan 2 for confocal imaging. The new Multiplex mode allows superresolution 3D imaging with up to 1.5× higher resolution. Additionally, researchers can easily separate multiple labels with spectral imaging. New users and multiuser facilities especially enjoy the system's inbuilt automation and practical features when setting up complex experiments. They can expect better data in a shorter time, with less training and maintenance. As users' requirements grow, they can expand ZEISS Celldiscoverer 7 with confocal technology, external cameras, deconvolution, and additional environmental control—whatever they need for the challenge of live-cell observation.

Superresolution Confocal Microscope

The next-generation SP8 LIGHTNING confocal microscope from Leica Microsystems offers five highly sensitive detection channels for superresolution live-cell imaging in multicolor. LIGHTNING marks a new level of image quality with superresolution in all five channels down to 120 nm, enabling researchers to resolve the finest details in all color channels at once. For easy navigation of complex samples, LAS X Navigator software enables correlation of high-resolution images with overview images. In addition, scientists can combine and accommodate different research methods with the SP8 LIGHTNING, ranging from confocal microscopy with superresolution to stimulated emission depletion (STED) nanoscopy. Methods such as multiphoton microscopy, lifetime imaging, light-sheet imaging, or coherent anti-Stokes Raman scattering (CARS) can also be added, further expanding the research possibilities.

Tissue-Clearing Kits and Reagents

Abcam's tissue-clearing kits and reagents enable easy, rapid clearing for 3D imaging of tissues and cell culture models. Traditionally, 3D imaging of thick tissue sections and 3D cell culture models (organoids, microtissues, spheroids) has presented many technical challenges, including laborious and time-consuming tissue processing, layer-by-layer 2D imaging of thin sections, and in silico 3D reconstruction. By contrast, tissue-clearing reagents can expedite detailed 3D imaging by reducing the need for these steps, while providing a truer reflection of biological structures. Developed with Visikol's HISTO technology, Abcam's tissue-clearing kits use simple protocols and standard laboratory equipment, and are compatible with immunostaining, fluorescent protein, and chemical dyes. Clearing is also reversible for conventional H&E or IHC staining following 3D imaging. A selection of recombinant antibodies for key markers (NeuN, S100, and Iba1) have been validated for use with the kits. For greater flexibility, tissue-clearing reagents can be purchased as part of a kit or individually.

Glass-Bottom Microplates

The family of ultraflat Krystal glass-bottom microplates from Porvair Sciences provides unmatched performance for high-throughput screening and tissue culture applications. Krystal plates combine the advantageous optical properties of glass—low background and low birefringence—with the versatility of a microplate. Precision-engineered using a polystyrene frame and a borosilicate glass sheet fixed to the base with a biocompatible adhesive, these plates offer a high degree of planar flatness (+/–50 μm) across the base, excellent light transmission, and a flat optical plane for growing cells. In addition, the nominal cutoff wavelength of 335 nm allows most fluorescence assays to be excited or read through the plate glass bottom. Together, these unique advantages translate into a significant increase in measurement precision and elimination of read errors when performing cell-based assays using fluorescent or luminescent imaging. Krystal plates are available in a choice of ANSI/SLAS standard 96- and 384-well formats.

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