Technical Comments

Comment on “Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry”

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Science  08 Nov 2019:
Vol. 366, Issue 6466, eaaw9830
DOI: 10.1126/science.aaw9830


Chorev et al. (Reports, 16 November 2018, p. 829) describe mass spectrometry on mitochondrial membrane proteins ionized directly from their native environment. However, the assignments made to measured masses are incorrect or inconclusive and lack experimental validation. The proteins are not in their “native” condition: They have been stripped of tightly bound lipids, and the complexes are fragmented or in physiologically irrelevant oligomeric states.

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