Technical Comments

Response to Comment on “Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry”

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Science  08 Nov 2019:
Vol. 366, Issue 6466, eaax3102
DOI: 10.1126/science.aax3102

Abstract

Hirst et al. claim that proteins ejected directly from mitochondrial membranes in our study are degraded, are incorrectly assigned, lack lipids, and show discrepancies with “native states” mostly obtained in detergent micelles. Here, we add further evidence in full support of our assignments and show that all complexes are either ejected intact or in known intermediate states, with core subunit interactions maintained. None are degraded or rearranged.

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