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Comment on “Ancient origins of allosteric activation in a Ser-Thr kinase”

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Science  20 Nov 2020:
Vol. 370, Issue 6519, eabc8301
DOI: 10.1126/science.abc8301


Hadzipasic et al. (Reports, 21 February 2020, p. 912) used ancestral sequence reconstruction to identify historical sequence substitutions that putatively caused Aurora kinases to evolve allosteric regulation. We show that their results arise from using an implausible phylogeny and sparse sequence sampling. Addressing either problem reverses their inferences: Allostery and the amino acids that confer it were not gained during the diversification of eukaryotes but were lost in a subgroup of Fungi.

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