Micro-Optical Sectioning Tomography to Obtain a High-Resolution Atlas of the Mouse Brain

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Science  04 Nov 2010:
DOI: 10.1126/science.1191776


The neuroanatomical architecture is considered to be the basis for understanding brain functions and dysfunctions. However, existing imaging tools have limitations for brainwide mapping of neural circuits at a mesoscale level. We developed a Micro-Optical Sectioning Tomography (MOST) system that can provide micron tomography of a centimeter-sized whole mouse brain. Using MOST, we obtained a 3D structural dataset of a Golgi-stained whole mouse brain at the neurite level. The morphology and spatial locations of neurons and traces of neurites can be clearly distinguished. We found that neighboring Purkinje cells are sticking to each other.