Supplemental Data
![]() ![]() | A Sperm Cytoskeletal Protein That Signals Oocyte Meiotic Maturation and Ovulation M.A. Miller, V.Q. Nguyen, M.-H. Lee, M. Kosinski, T. Schedl, R.M. Capriloli, D. Greenstein |
Supplementary Material
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Movies. Analysis of MSP signaling by time-lapse video microscopy (1/12 real time). For best viewing, set the monitor color depth on grays.
- Movie 1
Wild type ovulation.mov (1.8 MB). Oocyte maturation and ovulation in a wild-type hermaphrodite, the first ovulation in the gonad arm is shown. - Movie 2
fog-2 mock injection.mov (1.1 MB). A buffer-injected fog-2(q71) female showing a low sheath cell contraction rate. - Movie 3
fog-2 MSP injection #1.mov (872 K). Oocyte maturation and ovulation in a fog-2(q71) female injected with sperm-purified MSP. Note that strong sheath contractions continue following ovulation, unlike the situation in the wild type. - Movie 4
fog-2 MSP injection #2.mov (1.4 MB). A fog-2(q71) female injected with sperm-purified MSP showing an increase in the rate and intensity of sheath cell contractions.
- Movie 1
Methods
Phylogenetic analyses were performed using maximum parsimony and neighbor-joining methods. Amino acid sequences from MSP and MSP-like domains of several representative VAPs were used in the analyses. For parsimony, the heuristic search option of PAUP* 3.1 (1) was used for tree construction, with 200 random order taxon addition replicates and tree bisection and reconnection branch swapping. Bacterial PapD, which is structurally related to MSP, was used as the outgroup. The "protpars" matrix of PAUP* 3.1 was used to weigh amino acid substitutions. To obtain bootstrap values, 100 bootstrap replicates were performed using simple taxon addition with tree bisection and reconnection branch swapping.
References
1. D. Swofford, PAUP: Phylogenetic analysis using parsimony (Illinois National History Survey, Champaign, IL, 1993).