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HIFα Targeted for VHL-Mediated Destruction by Proline Hydroxylation: Implications for O2 Sensing
Mircea Ivan, Keiichi Kondo, Haifeng Yang, William Kim, Jennifer Valiando, Michael Ohh, Adrian Salic, John M. Asara, William S. Lane, William G. Kaelin Jr.

Supplementary Material

Supplemental Figure 1. Confirmation of Pro564 hydroxylation by MS/MS using microcapillary HPLC directly coupled to a Finnegan LCQ quadrupole ion trap mass spectrometer. Targeted ion MS/MS (TIMM) of the doubly protonated ion at m/z 1267 of the HIF (556-575) peptide was performed with an isolation width of 2.5 daltons and relative collision energy of 30%.

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