Supporting Online Material


Abstract
Full Text
Oscillations in NF-κB Signaling Control the Dynamics of Gene Expression
D. E. Nelson et al.

Supporting Online Material

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This supplement contains:

Materials and Methods
Figs. S1 to S19
Table S1
References

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  • Movie S1
    Time-lapse confocal images of SK-N-AS cells expressing RelA-DsRed (red) and hCMV- IκBα-EGFP (green) over a period of 10 h following continual stimulation with 10 ng/ml TNFα(see Figure 1B and 2A). Images were taken at 3 min intervals.
  • Movie S2
    Time-lapse confocal images of SK-N-AS cells expressing RelA-DsRed (red) and EGFP (green) over a period of 10 h following continual stimulation with 10 ng/ml TNFα(see Figure 2C). Images were taken at 3 min intervals.
  • Movie S3
    Time-lapse luminescence images of HeLa and SK-N-AS cells expressing pNF-κB-Luc over a period of 25 h after continual stimulation with TNFa (see Figure S11). Images were taken at 30 min intervals (30 min integrations).
  • Movie S4
    Time-lapse confocal images of HeLa cells expressing RelA-DsRed-Express and κB-IκBα-EGFP over a period of 15 h following continual stimulation with 10ng/ml TNFα (see Fig. 3A and B). Images were taken at 3 min intervals.