Supporting Online Material


B Cell Ligand Discrimination Through a Spreading and Contraction Response
S. J. Fleire, J. P. Goldman, Y. R. Carrasco, M. Weber, D. Bray, F. D. Batista

Supporting Online Material

This supplement contains:
Materials and Methods
SOM Text
Figs. S1 to S5
Table S1
References
Movies S1 to S4

On 12 May 2006, Figs. S1 to S5 were replaced with figures of higher quality and readability.

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Movie S1
This video shows the time-lapse reconstruction of the kinetics of HELWT antigen aggregation (density = 150 molecules/μm2) as derived from the Brownian dynamics simulation. Single frames were obtained from the simulations every 10 s and assembled into a movie.

Movie S2
This video shows a time-lapse reconstruction of the kinetics of HELWT antigen aggregation (density = 150 molecules/μm2) as measured in vitro (left panel) and as derived from the in silico simulation (right panel). Single frames were obtained from the simulations every 60 s and assembled into a movie.

Movie S3
This video shows the time-lapse comparison of the antigen aggregation during Brownian dynamics simulation at varying densities: 150 molecules/μm2 (left panel), 75 molecules/μm2 (central panel) and 15 molecules/μm2 (right panel) of antigen. Single frames were obtained from the simulations every 60 s and assembled into a movie.

Movie S4
This video shows the time-lapse comparison of the antigen aggregation during Brownian dynamics simulation when the affinity of the BCR for the antigen was: Ka= 2 x 1010 M-1 (left panel), Ka= 5.8 x 106 M-1 (central panel) and Ka= 1.4 x 106 M-1 (right panel) of antigen. Single frames were obtained from the simulations every 60 s and assembled into a movie.

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