Supporting Online Material

Reversal of Neurological Defects in a Mouse Model of Rett Syndrome
Jacky Guy, Jian Gan, Jim Selfridge, Stuart Cobb, Adrian Bird

Supporting Online Material

This supplement contains:
Materials and Methods
Figs. S1 to S7
Movies S1 to S3

This file is in Adobe Acrobat PDF format.

Movie S1
The movie shows a Mecp2lox-Stop/y,cre-ER mouse (#286, see Figure 2D) with neurological symptoms at 12 weeks of age. Note low stance, inertia, tremor, arrhythmic breathing, splayed hind limb position, and moderate hindlimb clasping. TM injection was initiated on this day.

Movie S2
The same mouse as shown in Movie S1 four weeks later after a course of five weekly TM injections.

Movie S3
Female mice that received identical TM administration regimes 26 weeks prior to filming. The first mouse seen is a Stop/+,cre female that displayed symptoms at the beginning of TM treatment and is now indistinguishable from wild-type using our symptom scoring system (see Materials and Methods). The second mouse entering the frame is a wild-type female. The third mouse to appear is a Stop/+ female lacking the cre-ER transgene, which therefore fails to respond to TM. Note inertia and obesity of this third mouse.

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