Supporting Online Material


A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity
Chava Kimchi-Sarfaty, Jung Mi Oh, In-Wha Kim, Zuben E. Sauna, Anna Maria Calcagno, Suresh V. Ambudkar, Michael M. Gottesman

Supporting Online Material

This supplement contains:
Materials and Methods
Figs. S1 to S5
Table S1
References

Clarification (30 November 2007):
Based on an inquiry from Jack Kornblatt, the authors wish to clarify that the protein sequence was obtained from a detailed mass spectrometric study performed at the Harvard Microchemistry Facility (HMF) by microcapillary reverse-phase HPLC nano-electrospray tandem mass spectrometry. HMF performed both chymotryptic and pronase digestions of the protein. In all, 82 peptides (representing 37% of the Pgp sequence by amino acid count) were identified and sequenced (see appended figure and accompanying legend, pages 16 and 17). Each of these sequences was identical to the sequence of haplotype Pglycoprotein. Moreover, several different peptides encoded by the synonymous SNP (3435C>T), which is the key polymorphism linked to the functional change in Pgp, were sequenced and found to be unchanged. In addition, the analysis of codon usage, table S1 in the original Supporting Online Material (pages 2 to 15) contains for each codon around the three polymorphisms the frequency of this codon per 1000 codons in the human genome instead of RSCU values as stated in the text. These values were obtained from the codon usage Web site at (www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=9606). Figure 1, panels D to F, shows results in the presence of cyclosporin A (+CsA) not (+/-CsA) as indicated in the body of the figure. This is correctly stated in the legend. These clarifications do not affect the conclusions of the paper.
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