Supporting Online Material

Unidirectional Airflow in the Lungs of Alligators
C. G. Farmer and Kent Sanders

Supporting Online Material

This supplement contains:
Materials and Methods
Fig. S1

This file is in Adobe Acrobat PDF format.

Other Supporting Online Material for this manuscript includes the following:
Movies S1 to S3

Movies S1 and S2
Microspheres flowing through the cervical ventral bronchus (green bronchus of Fig. 1). In the cervical ventral bronchus most of the microspheres move in a cranial to caudal direction (from the right side of the image toward the left) as fluid is injected into the lung (Movie S1) and when fluid is withdrawn (Movie S2). A few microspheres are no longer suspended in the fluid and are caught in lung tissue. These move at a slower speed and spread apart as the injection takes place and move closer together as fluid leaves the lung. The black line in the lower left visual field is a piece suture on the outside of the lung.

Movie s3
Microspheres flowing through parabronchi. In this movie numerous microspheres have been caught in lung tissue and can be seen moving to the right when fluid is injected and to the left when it is withdrawn and then back to the right as fluid is injected again, which occurs within the same movie. Other microspheres remain suspended in the fluid and move at a greater speed from a dorsocranial (top of the screen) to ventrocaudal direction (bottom of the screen) as saline was injected and as it was withdrawn. These latter, faster moving microspheres do not move in a tidal pattern but flow unidirectionally.

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