Supporting Online Material


Cortical Constriction During Abscission Involves Helices of ESCRT-III—Dependent Filaments
Julien Guizetti, Lothar Schermelleh, Jana Mäntler, Sandra Maar, Ina Poser, Heinrich Leonhardt, Thomas Müller-Reichert, Daniel W. Gerlich

Supporting Online Material

This supplement contains:
Materials and Methods
SOM Text
Figs. S1 to S12
References

This file is in Adobe Acrobat PDF format.

Other Supporting Online Material for this manuscript includes the following:

Movie s1
Microtubule dynamics during cytokinesis. Confocal timelapse imaging of HeLa cell expressing EGFP-α-tubulin (maximum intensity projection of 12 z-slices). Time-lapse is 4 min.

Movie s2
Kinetics of CHMP4B-EGFP localization at the intercellular bridge. Single z-sections from 3D-confocal time-lapse movie of HeLa cell stably expressing CHMP4B-EGFP (green) and mRFP-α-tubulin (red). Timelapse: 6.5 min.

Movie s3
3D-SIM of mid-stage intercellular bridge. HeLa cell stably expressing CHMP4B-EGFP (green) was stained with anti-tubulin antibody (red). The movie shows maximum intensity projections rotated on two axes.

Movie s4
3D-SIM of late stage intercellular bridge. HeLa cell stably expressing CHMP4B-EGFP (green) was stained with anti-tubulin antibody (red). The movie shows maximum intensity projections rotated on two axes.

Movie s5
Tomographic z-sections of intercellular bridge at mid abscission stage. Z-section spacing is 1.5 nm.

Movie s6
Model of the abscission structure based on merged tomographic semi-thin sections of late-stage abscission site. Red: microtubules; green: cortical 17 nm filaments (three cortical filaments are labeled by different tones of green); gray: midbody; white balls: open microtubule ends; blue balls: closed microtubule ends. The plasma membrane is not displayed for better visualization of internal structures.

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