Supporting Online Material

Traffic Jams Reduce Hydrolytic Efficiency of Cellulase on Cellulose Surface

Kiyohiko Igarashi, Takayuki Uchihashi, Anu Koivula, Masahisa Wada, Satoshi Kimura, Tetsuaki Okamoto, Merja Penttilä, Toshio Ando, Masahiro Samejima

Materials/Methods, Supporting Text, Tables, Figures, and/or References

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  • Materials and Methods
  • References (27-33)

Images, Video, and Other Other Media

Movie S1
HS-AFM images of TrCel7A (final conc. 2.0 μM) sliding on crystalline cellulose Iα. x/y=150/90 nm, 10x playback, total time 153 s. Scale bar and height lookup table (LUT) of the images are shown in Fig. 1A.
Movie S2
HS-AFM images of TrCel7A (final conc. 2.0 μM) on cellulose IIII. x/y=180/100 nm, 10x playback, total time 466 s. Scale bar and height LUT of the images are shown in Fig. 2C.
Movie S3
HS-AFM images of TrCel7A (final conc. 2.0 μM) on cellulose IIII. x/y=72/40 nm, 5x playback, total time 154 s. Scale bar and height LUT of the images are included in the images.
Movie S4
HS-AFM images of TrCel7A (final conc. 2.0 μM) on cellulose IIII. x/y=150/75 nm, 10x playback, total time 90 seconds. Scale bar and height LUT of the images are shown in Fig. 3B.
Movie S5
HS-AFM images of TrCel7A (final conc. 2.0 μM) on cellulose IIII peeling the surface of the same cellulose fiber of movie S4. x/y=150/75 nm, 10x playback, total time 372 seconds. Scale bar and height LUT of the images are shown in Fig. 3B.
Movie S6
HS-AFM images of cellulose IIII before the enzyme addition. x/y=250/125 nm, 10x playback, total time 18 seconds
Movie S7
HS-AFM images of crystalline cellulose IIII with the addition of TrCel6A (10 s) and TrCel7A (505 s) with final concentrations of 2.0 μM each. x/y=250/125 nm, 10x playback, total time 832 seconds.
Movie S8
Three-dimensional HS-AFM images of cellulose IIII incubated with TrCel6A. This movie was created from the first 480 s of movie S7. TrCel6A was added (10 s) at a final concentration of 2.0 μM. Height LUT of these images is shown in Fig. 4.
Movie S9
Three-dimensional HS-AFM images of cellulose IIII incubated with TrCel6A+TrCel7A. This movie was created from the latter part of movie S7. After 495 s from the addition of TrCel6A (5 s from the initial frame in this movie), TrCel7A was added at a final concentration of 2.0 μM.