Supporting Online Material

X-ROS Signaling: Rapid Mechano-Chemo Transduction in Heart

Benjamin L. Prosser, Christopher W. Ward, W. J. Lederer

Materials/Methods, Supporting Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S11
  • References (2931)
Correction (12 September 2011): A line stating where MyoTak can be obtained has been added to the section "Cardiomyocyte attachment and stretch," on page 1 of Materials and Methods.
The original version can still be accessed here.

Images, Video, and Other Other Media

Movie S1
Stretch of single rat-ventricular myocyte. An intact single ventricular myocyte is attached to stiff glass micro-rods via MyoTakTM. The cell is then subjected to a series of small step like changes in length occurring every few seconds (video speed 4x).
Movie S2
3D reconstruction of myocyte attached to fluorescent MyoTak. Single ventricular myocyte stained with Di-8-ANEPPS to label the sarcolemma and t-tubules is attached to glass micro-rods via MyoTak. The MyoTak is fluorescent and is evident as a coating around the micro-rods. There is a large surface area of interaction between MyoTak and the cell membrane, which promotes robust cell attachment and control of cell length.