Supplementary Materials

ESCRT-III Governs the Aurora B-Mediated Abscission Checkpoint Through CHMP4C

Jeremy G. Carlton, Anna Caballe, Monica Agromayor, Magdalena Kloc, Juan Martin-Serrano

Materials/Methods, Supporting Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S9
  • Full References

Images, Video, and Other Other Media

Movie S1
Asynchronous cultures of HeLa mCh-Tub cells were transfected with nontargeting siRNA and imaged live by using a 40x objective acquiring 3 x 1.25 μm–spaced Z-positions. Frames collected every 10 minutes. A 200-ms delay was introduced between each frame in presented movie
Movie S2
Asynchronous cultures of HeLa mCh-Tub cells were transfected with Luciferase siRNA and imaged live by using a 40x objective acquiring 3 x 1.25 μm–spaced Z-positions. Frames collected every 10 minutes. A 200-ms delay was introduced between each frame in presented movie.
Movie S3
Asynchronous cultures of HeLa mCh-Tub cells were transfected with CHMP4C-1 siRNA and imaged live by using a 40x objective acquiring 3 x 1.25 μm–spaced Z-positions. Frames collected every 10 minutes. A 200-ms delay was introduced between each frame in presented movie.
Movie S4
Asynchronous cultures of HeLa mCh-Tub cells were transfected with CHMP4C-2 siRNA and imaged live by using a 40x objective acquiring 3 x 1.25 μm–spaced Z-positions. Frames collected every 10 minutes. A 200-ms delay was introduced between each frame in presented movie.
Movie S5
Asynchronous cultures of HeLa cells stably expressing mCh-Tubulin and GFP-CHMP4B were imaged live by using a 60x objective. 5 x 1.5 μm–spaced Z-positions were collected every 10 minutes. A 200-ms delay was introduced between each frame in presented movie.
Movie S6
Asynchronous cultures of HeLa cells stably expressing mCh-Tubulin and GFP-CHMP4C were imaged live by using a 60x objective. 5 x 1.5 μm–spaced Z-positions were collected every 10 minutes. A 200-ms delay was introduced between each frame in presented movie.
Movie S7
Asynchronous cultures of HeLa YFP-LAP2β cells were transfected with Luciferase siRNA and imaged live by using a 40x objective acquiring 3 x 1.25 μm–spaced Z-positions. Frames collected every 10 minutes. A 200-ms delay was introduced between each frame in presented movie.
Movie S8
Asynchronous cultures of HeLa YFP-LAP2β cells were transfected with CHMP4C-1 siRNA and imaged live by using a 40x objective acquiring 3 x 1.25 μm–spaced Z-positions. Frames collected every 10 minutes. A 200-ms delay was introduced between each frame in presented movie.
Movie S9
Asynchronous cultures of HeLa YFP-LAP2β cells were transfected with CHMP4C-2 siRNA and imaged live by using a 40x objective acquiring 3 x 1.25 μm–spaced Z-positions. Frames collected every 10 minutes. A 200-ms delay was introduced between each frame in presented movie.