Supplementary Materials

A Papaver somniferum 10-Gene Cluster for Synthesis of the Anticancer Alkaloid Noscapine

Thilo Winzer, Valeria Gazda, Zhesi He, Filip Kaminski, Marcelo Kern, Tony R. Larson, Yi Li, Fergus Meade, Roxana Teodor, Fabián E. Vaistij, Carol Walker, Tim A. Bowser, Ian A. Graham

Materials/Methods, Supporting Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S10
  • Tables S1, S2, S3, S4, and S7
  • Legends for tables S5, S6, S8, and S9
  • References
Table S5
HN1 401Kb gene cluster region annotation (provided as separate Excel file).
(A) Positional information and type of ORFs predicted by the FGENESH gene
prediction program (42) within the HN1 gene cluster genomic region. (B) Positional
information and type of repetitive elements predicted by the RepeatMasker program
( within the HN1 gene
cluster genomic region. (C) Summary repetitive elements predicted by RepeatMasker.
All positional information refers to the HN1 gene cluster genomic region consensus
sequence provided as a text file in Table S9.
Table S6
Elements identified in the promoter region of the ten cluster genes (provided
as separate Excel file). The 1Kb upstream region relative to the start of translation
for each gene was compared against the PLACE database (20).
Table S8
Primer sequences and associated information (provided as separate Excel file).
Table S9
Text file of the 401 Kb region of genomic DNA depicted in Fig. 3 and detailed in
Table S5.
Gaps representing short highly repetitive regions are denoted by a sequence
of 100 Ns and are detailed further in the NCBI accessions.