Supplementary Materials

The Cells and Circuitry for Itch Responses in Mice

Santosh K. Mishra, Mark A. Hoon

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S4
  • Tables S1 and S2
  • References (2225)

Images, Video, and Other Other Media

Movie S1
Movie S1. Intradermal injection of histamine in wild type and Nppb−/− mice.Intradermal injection of the puritogen histamine into the nape of the neck. Video shows the typical scratch response of wild type animals that begin approx. 5 min after injection and continue for about 25-30 minutes. As illustrated in the video Nppb−/− mice exhibit dramatically reduced scratch responses compared to wild-type littermates.
Movie S2
Movie S2. Intrathecal injection of Nppb in wild type and Nppb−/− mice.Intrathecal injection of Nppb evokes scratching in mice starting about 5 minutes after stimulation and lasting approximately 30 minutes. Both wild type and Nppb−/− mice exhibit equivalent responses that closely resemble responses of wild type mice to pruitogens.
Movie S3
Movie S3. Intrathecal injection of Substance P in wild type and Nppb−/− mice.For comparison, we also injected the nociceptive neuropeptide Substance P intrathecally. Substance P elicits a profoundly different behavioral response. Video illustrates the responses of wild type and Nppb−/− mice. After Substance P injection, we never observed scratching but instead noted typical nociceptive withdrawal behavior (freezing, hunched posture and spasmodic twitching) with rapid onset and approx. 10 min duration.