Supplementary Materials

Identification of a Colonial Chordate Histocompatibility Gene

Ayelet Voskoboynik, Aaron M. Newman, Daniel M. Corey, Debashis Sahoo, Dmitry Pushkarev, Norma F. Neff, Benedetto Passarelli, Winston Koh, Katherine J. Ishizuka, Karla J. Palmeri, Ivan K. Dimov, Chen Keasar, H. Christina Fan, Gary L. Mantalas, Rahul Sinha, Lolita Penland, Stephen R. Quake, Irving L. Weissman

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S21
  • Tables S1 to S4 and S7 to S9
  • Legends for Tables S5 and S6
  • Full References

Images, Video, and Other Other Media

Movie S1
Fusion and rejection under morpholino control treatment.. Colony 1 was injected with ˜3μl standard control Vivo-Morpholino oligonucleotides and a colony allorecognition assay (CAA) was arranged between the treated colony and 2 young colonies (3 weeks old; colony 2 and 3). Two days following CAA setup and initial treatment, 3 rejection points (brown/black spots) appeared on the ampullae border between colonies 1 and 2. At +3 days, a vasculature fusion was established between colonies 1 and 3. This movie, taken at +3 days, contrasts the common blood flow shared by colonies with a fused vasculature (colonies 1 and 3) with the isolated blood systems and points of rejection that characterize histoincompatible colonies (colonies 1 and 2).
Movie S2
Unreactive colonies treated with BHF splice-inhibiting morpholinos. Colony 1 was injected with 3μl BHF splice-inhibiting Vivo-Morpholino oligonucleotides. That same day, a colony allorecognition assay was arranged between colony 1 and two young colonies, both progeny of the same wildtype parent (3 weeks old). Despite establishing physical ampullae contact, all colonies remained unreactive, even five days following initial treatment. This movie was taken at +3 days. Notice two orange ampullae protruding from colony 1 that have interfaced with colony 2. Despite close physical contact, no fusion/rejection reaction occurred.
Table S5
B. schlosseri gene sequences analyzed in this work and fosmid sequence.
Table S6
Details of analyzed B. schlosseri genes, including concordance with fusibility outcomes and genetically defined lines.