Supplementary Materials

The Molecular Trigger for High-Speed Wing Beats in a Bee

H. Iwamoto, N. Yagi

Materials/Methods, Supporting Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S3
  • Tables S1
  • Full References

Images, Video, and Other Other Media

Movie S1
Movie of the wing-beat of a bumblebee, containing a whole wing-beat cycle. The
yellow dot indicates the wing position determined by software.
Movie S2
Example of X-ray diffraction movie from IFMs of a beating bumblebee, containing a
whole wing-beat cycle. The frames have been edited by the method shown in fig.
S1, but the orientation of the patterns are left unchanged. The change in the
orientations of the muscle fiber axes can be observed.
Movie S3
Annotated version of movie S2. The lines indicate the axes of the diffraction pattern.
Solid line, equator; broken line, meridian. The circles indicate the expected positions
of some reflections. The numbers 1 and 2 denote the 1,0,1 and 1,0,2 reflection spots,
respectively, and M denotes the 1st myosin meridional reflection. Blue, DLM;
green, DVM.
Movie S4
X-ray diffraction movie from nine bumblebees, with equator of DLM horizontal. The
diffraction patterns from nine bumblebees were summed after reorienting so that the
equator of DLM was horizontal, and folding the 1st and 2nd quadrants to the 3rd and
4th quadrants, respectively. The regions of interest, boxed in cyan lines, are magnified
on the upper left. The slide bar on the left shows the sarcomere length change
estimated from the lattice spacing. Because DVM fibers make various angles with
respect to DLM fibers, the reflections from DVM are blurred.
Movie S5
X-ray diffraction movie from nine bumblebees, with equator of DVM horizontal. The
diffraction patterns from nine bumblebees were summed after reorienting so that the
equator of DVM was horizontal, and folding the 1st and 2nd quadrants to the 3rd
and 4th quadrants, respectively. The arrangement is a mirror image of movie S4. In
this movie, the reflections from DLM are blurred.