Supplementary Materials

Genomically Recoded Organisms Expand Biological Functions

Marc J. Lajoie, Alexis J. Rovner, Daniel B. Goodman, Hans-Rudolf Aerni, Adrian D. Haimovich, Gleb Kuznetsov, Jaron A. Mercer, Harris H. Wang, Peter A. Carr, Joshua A. Mosberg, Nadin Rohland, Peter G. Schultz, Joseph M. Jacobson, Jesse Rinehart, George M. Church, Farren J. Isaacs

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S22
  • Tables S1 to S37
  • References (2670)
Table S3
Summaries of mutations observed in each strain of recoding lineage. (Excel)
Table S4
All mutations observed in recoded strain lineage. (Excel)
Table S16
UAG codons converted to UAA codons in each strain of recoding lineage. (Excel)
Table S19
DNA oligonucleotides used in this study. (Excel)
Table S26
Putative MAGE oligo mistargeting events. (Excel)
Table S27
All uncharacterized Pindel breakpoint events. (Excel)
Table S28
All complete Pindel structural events. (Excel)
Table S29
All high quality Breakdancer events. (Excel)
Table S31
Sequencing coverage of genome in each strain of recoding lineage. (Excel)
Table S32
Summary of CAGE lineage for removing all instances of UAG from a single genome. (Excel)
Table S34
List of all 321 targeted UAG locations in MG1655. (Excel)
Table S35
List of cassette insertions and structural events used to generate C321.ΔA Genbank annotation . (Excel)
Table S36
List of variants used to generate C321.ΔA strain. (Excel)