Supplementary Materials
Hedgehog Signaling Controls T Cell Killing at the Immunological Synapse
Maike de la Roche, Alex T. Ritter, Karen L. Angus, Colin Dinsmore, Charles H. Earnshaw, Jeremy F. Reiter, Gillian M. Griffiths
Materials/Methods, Supplementary Text, Tables, Figures, and/or References
- Materials and Methods
- Figs. S1 to S6
- Full Reference List
Images, Video, and Other Other Media
- Movie showing OT-I CTL transiently expressing Smo-EGFP and PACT-RFP (centrosome) conjugating with EL4 target cell (blue). Serial, confocal Z-stacks taken every 20 s. Movie runs at 6 frames/s.
- Movie showing carrier-only-treated OT-I CTL transiently expressing LifeAct-EGFP (actin) and PACT-RFP (centrosome) conjugating with EL4 target cell (blue). Serial, confocal Zstacks, taken every 25 s. Image periodically rotated 90 degrees to demonstrate actin clearance from immunological synapse.
- Movie showing cyclopamine-treated OT-I CTL transiently expressing LifeAct-EGFP (actin) and PACT-RFP (centrosome) conjugating with EL4 target cell (blue). Serial, confocal Zstacks, taken every 25 s. Image periodically rotated 90 degrees to demonstrate actin organization at the immunological synapse.
