Supplementary Materials

Hedgehog Signaling Controls T Cell Killing at the Immunological Synapse

Maike de la Roche, Alex T. Ritter, Karen L. Angus, Colin Dinsmore, Charles H. Earnshaw, Jeremy F. Reiter, Gillian M. Griffiths

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

Download Supplement
  • Materials and Methods
  • Figs. S1 to S6
  • Full Reference List

Images, Video, and Other Other Media

Movie S1
Movie showing OT-I CTL transiently expressing Smo-EGFP and PACT-RFP (centrosome) conjugating with EL4 target cell (blue). Serial, confocal Z-stacks taken every 20 s. Movie runs at 6 frames/s.
Movie S2
Movie showing carrier-only-treated OT-I CTL transiently expressing LifeAct-EGFP (actin) and PACT-RFP (centrosome) conjugating with EL4 target cell (blue). Serial, confocal Zstacks, taken every 25 s. Image periodically rotated 90 degrees to demonstrate actin clearance from immunological synapse.
Movie S3
Movie showing cyclopamine-treated OT-I CTL transiently expressing LifeAct-EGFP (actin) and PACT-RFP (centrosome) conjugating with EL4 target cell (blue). Serial, confocal Zstacks, taken every 25 s. Image periodically rotated 90 degrees to demonstrate actin organization at the immunological synapse.