Supplementary Materials

PCP and Septins Compartmentalize Cortical Actomyosin to Direct Collective Cell Movement

Asako Shindo, John B. Wallingford

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S14
  • References

Images, Video, and Other Other Media

Movie S1
Time-lapse movie of a Keller explant undergoing CE. The cell membrane is marked by membrane-GFP, and v-junctions possessing a small neighboring vertex angle (ϕ) are colored magenta. Note that most of cells show rearrangement after the v-junctions finish shrinking. Time-lapse spans 110min, with images at 30 second intervals.
Movie S2
Time-lapse movie of a Keller explant. Highlighted cells show the association of v-junction shrinkage with multiple rounds of cell rearrangement (see also Fig. S4). The red lines indicate shrinking v-junctions. The cell membranes are marked by membrane-GFP. Time-lapse spans 141 min, with images at 30 second intervals.
Movie S3
Laser ablation of a short v-junction in a control explant. Membrane-GFP shows the cell outline. Vertex position exhibits a significant shift after ablation.
Movie S4
Laser ablation of a short t-junction in a control explant. Membrane-GFP shows the cell outline. Vertex position does not exhibit a significant shift after ablation.
Movie S5
Mosaic expression of Utrophin-GFP and Utrophin-RFP in Fig. 2. GFP expression distinguishes bi-polar lamellipodia (left), and RFP shows actin at the v-junction (right). The movie is played twice, in the second playing, arrows indicate actin accumulation at the v-junction.