Supplementary Materials

Nucleocytoplasmic Shuttling of a GATA Transcription Factor Functions as a Development Timer

Huaqing Cai, Mariko Katoh-Kurasawa, Tetsuya Muramoto, Balaji Santhanam, Yu Long, Lei Li, Masahiro Ueda, Pablo A. Iglesias, Gad Shaulsky, Peter N. Devreotes

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Figs. S1 to S10
  • Tables S1 and S2
  • Captions for Movies S1 to S11

Images, Video, and Other Other Media

Movie S1
GFP-GtaC exhibits oscillatory nucleocytoplasmic shuttling during early development. Images were captured at 30 sec intervals and played back at 10 fps. Corresponds to Fig. 1A and fig. S2A.
Movie S2
Localization of GFP-GtaC in 7 h post-starvation cells. Images were captured at 30 sec intervals and played back at 5 fps.
Movie S3
Wave-like spreading of the nuclear localization of GFP-GtaC. Images were captured at 1 min intervals and played back at 4 fps. Corresponds to fig. S3.
Movie S4
The nucleus-to-cytoplasm translocation of GFP-GtaC in response to uniform cAMP stimulation. Images were captured at 20 sec intervals and played back at 4 fps. Corresponds to the first panel in Fig. 1D.
Movie S5
Translocation GFP-GtaC from the cytoplasm into the nucleus when uniform cAMP stimulus was removed. Images were captured at 20 sec intervals and played back at 4 fps. Corresponds to the second panel in Fig. 1D.
Movie S6
The nucleus-to-cytoplasm translocation of GFP-GtaC was blocked in car1−car3/minus;cells. Images were captured at 20 sec intervals and played back at 4 fps. Corresponds to the third panel in Fig. 1D.
Movie S7
The nucleus-to-cytoplasm translocation of GFP-GtaCC-S in response to uniform cAMP stimulation. Images were captured at 20 sec intervals and played back at 4 fps. Corresponds to the fourth panel in Fig. 1D.
Movie S8
Translocation of NLSex-GFP-GtaCC-S in response to uniform cAMP stimulation. Images were captured at 20 sec intervals and played back at 4 fps. Corresponds to fig. S6A.
Movie S9
GFP-GtaC/gtaC cells starved for 2.5 h chemotaxing toward a micropipette releasing cAMP. Images were captured at 30 sec intervals and played back at 10 fps. Corresponds to Fig. 3A.
Movie S10
GFP-GtaC/gtaC cells starved for 4.5 h chemotaxing toward a micropipette releasing cAMP. Images were captured at 30 sec intervals and played back at 10 fps. Corresponds to Fig. 3A.
Movie S11
NLSex-GFP-GtaC/gtaC cells starved for 2.5 h chemotaxing toward a micropipette releasing cAMP. Images were captured at 30 sec intervals and played back at 10 fps. Corresponds to Fig. 3A.