Supplementary Materials
Nucleocytoplasmic Shuttling of a GATA Transcription Factor Functions as a Development
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Huaqing Cai, Mariko Katoh-Kurasawa, Tetsuya Muramoto, Balaji Santhanam, Yu Long, Lei Li, Masahiro Ueda, Pablo A. Iglesias, Gad Shaulsky, Peter N. Devreotes
Materials/Methods, Supplementary Text, Tables, Figures, and/or References
- Figs. S1 to S10
- Tables S1 and S2
- Captions for Movies S1 to S11
Images, Video, and Other Other Media
- GFP-GtaC exhibits oscillatory nucleocytoplasmic shuttling during early development. Images were captured at 30 sec intervals and played back at 10 fps. Corresponds to Fig. 1A and fig. S2A.
- Localization of GFP-GtaC in 7 h post-starvation cells. Images were captured at 30 sec intervals and played back at 5 fps.
- Wave-like spreading of the nuclear localization of GFP-GtaC. Images were captured at 1 min intervals and played back at 4 fps. Corresponds to fig. S3.
- The nucleus-to-cytoplasm translocation of GFP-GtaC in response to uniform cAMP stimulation. Images were captured at 20 sec intervals and played back at 4 fps. Corresponds to the first panel in Fig. 1D.
- Translocation GFP-GtaC from the cytoplasm into the nucleus when uniform cAMP stimulus was removed. Images were captured at 20 sec intervals and played back at 4 fps. Corresponds to the second panel in Fig. 1D.
- The nucleus-to-cytoplasm translocation of GFP-GtaC was blocked in car1−car3/minus;cells. Images were captured at 20 sec intervals and played back at 4 fps. Corresponds to the third panel in Fig. 1D.
- The nucleus-to-cytoplasm translocation of GFP-GtaCC-S in response to uniform cAMP stimulation. Images were captured at 20 sec intervals and played back at 4 fps. Corresponds to the fourth panel in Fig. 1D.
- Translocation of NLSex-GFP-GtaCC-S in response to uniform cAMP stimulation. Images were captured at 20 sec intervals and played back at 4 fps. Corresponds to fig. S6A.
- GFP-GtaC/gtaC− cells starved for 2.5 h chemotaxing toward a micropipette releasing cAMP. Images were captured at 30 sec intervals and played back at 10 fps. Corresponds to Fig. 3A.
- GFP-GtaC/gtaC− cells starved for 4.5 h chemotaxing toward a micropipette releasing cAMP. Images were captured at 30 sec intervals and played back at 10 fps. Corresponds to Fig. 3A.
- NLSex-GFP-GtaC/gtaC− cells starved for 2.5 h chemotaxing toward a micropipette releasing cAMP. Images were captured at 30 sec intervals and played back at 10 fps. Corresponds to Fig. 3A.
