Supplementary Materials

Motor skill learning requires active central myelination

Ian A. McKenzie, David Ohayon, Huiliang Li, Joana Paes de Faria, Ben Emery, Koujiro Tohyama, William D. Richardson

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S7
  • Captions for Movies S1 to S5
  • Full Reference List

Images, Video, and Other Other Media

Movie S1
Demyelination-induced tremor in S10-Myrf (-/-) mice
An S10-Myrf (-/-) mouse filmed five weeks post-tamoxifen (administered at P60), displaying general ataxia including tremor and momentary seizures typical of advanced de- or dys-myelination.
Movie S2
First introduction to the complex wheel (wt mouse)
A wild type mouse (C57Bl6/CBA F1 hybrid) was filmed during its first introduction to a complex wheel in low light conditions at the beginning of the dark cycle. The movie plays in slow-motion, one-tenth actual speed.
Movie S3
High-speed running on the complex wheel (wt mouse)
A wild-type mouse after seven days' exposure to a complex wheel (low-light conditions at the beginning of the dark cycle). The movie plays at actual speed, interrupted by freeze-frame sequences to show the foot stepping pattern (ipsilateral fore- and hind-paws grasp the same rungs).
Movie S4
P-Myrf (-/-) mouse on the complex wheel after training
A P-Myrf (-/-) mouse on the complex wheel after seven nights self-training. A slowmotion sequence (one-quarter actual speed) separates two sequences at actual speed. The mouse appears to run less spontaneously than its P-Myrf (+/-) littermate (movie S5). Although it is difficult to understand the precise problem from movies like this, it seems that placement of the hind paws in particular is less automatic and less accurate.
Movie S5
P-Myrf (+/-) mouse on the complex wheel after training A P-Myrf (+/-) mouse on the complex wheel after seven nights self-training. A slowmotion sequence (one-quarter actual speed) separates two actual-speed sequences.