Supplementary Materials

The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins

Mie Wong, Sean Munro

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

Download Supplement
  • Figs. S1 to S16
  • Captions for movies S1 and S2

Images, Video, and Other Other Media

Movie S1
C1-HeLa cells transiently expressing mitochondria-targeted mCherry-golgin-97ΔCterm- HA-MAO and pre-treated with 0.5 μM nocodazole for three hours to depolymerize microtubules prior to being incubated at 37°C for the indicated times with a mix of nocodazole and D/D solubilizer to induce secretion of the GFP-FM4-hGH reporter. Images were acquired every 2 minutes for 140 minutes. Elapsed time shown in minutes, scale bars 5 μm.
Movie S2
C1-HeLa cells transiently expressing mitochondria-targeted mCherry-GM130ΔCterm- HA-MAO and pre-treated with 0.5 μM nocodazole for three hours to depolymerize microtubules prior to being incubated at 37°C for the indicated times with a mix of nocodazole and D/D solubilizer to induce secretion of the GFP-FM4-hGH reporter. Images were acquired every 2 minutes for 140 minutes. Elapsed time shown in minutes, scale bars 5 μm.