Supplementary Materials

Time-restricted feeding attenuates age-related cardiac decline in Drosophila

Shubhroz Gill, Hiep D. Le, Girish C. Melkani, Satchidananda Panda

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S14
  • Captions for tables S1 to S9
  • Captions for Movies S1 to S5
  • References
Tables S1 to S9
Table S1. Genotypes of Drosophila melanogaster.
Table S2. List of starvation induced and suppressed genes identified by Farhadian et al, and respective fold change under TRF.
Table S3.
Expression pattern of transcripts in TRF that are known to change in adult Drosophila subjected to dietary restriction.
Table S4.
Transcripts scored circadian in 5 week old fly heads.
Table S5. Transcripts scored circadian in 5 week old fly periphery.
Table S6.
Differentially expressed transcripts in the heart of 5 week old flies under ALF and TRF regimens.
Table S7. Expression levels of components of mitochondrial electron transport chain in the myogenic heart of adult Drosophila under ALF or TRF regimens.
Table S8.
Expression levels of components of TRiC/CCT chaperonin components in the myogenic heart of adult Drosophilaunder ALF or TRF regimen.
Table S9. qPCR primers used in this study.

Images, Video, and Other Other Media

Movie S1
Representative videos of the ex vivo beating heart in Oregon-R (wildtype) male Drosophila maintained on the standard cornmeal medium at 3 weeks (left panels) and 5 weeks (right panels) of age under ALF (top panels) or TRF (bottom panels). Note the age-related deterioration of cardiac function from 3 to 5 weeks of age, especially the arrhythmias. Also, note that the 5 weeks old TRF flies have remarkably improved cardiac function compared to 5 weeks old ALF flies. Movie duration: 20 seconds, Frame-rate: 120–150 fps. Scale bar as shown therein.
Movie S2
Representative videos of the ex vivo beating heart in Oregon-R (wildtype) male Drosophila maintained on fat diet i.e. 2% coconut oil w/v + standard cornmeal medium at 5 weeks of age under ALF (top, FA) or TRF (bottom, FT). Note that the 5 weeks old FT flies have remarkably improved cardiac function compared to 5 weeks old FA flies. Movie duration: 15 seconds, Frame-rate: 120–150 fps. Scale bar as shown therein.
Movie S3
Representative videos of the ex vivo beating heart in circadian mutant clkar male Drosophila maintained on standard cornmeal medium at 5 weeks of age under ALF (top) or TRF (bottom). Movie duration: 15 seconds, Frame-rate: 120–150 fps. Scale bar as shown therein.
Movie S4
Representative videos of the ex vivo beating heart in TRiC subunit TCP1-Eta mutant male Drosophila maintained on standard cornmeal medium examined at 5 weeks of age under ALF (top) or TRF (bottom). Movie duration: 15 seconds, Frame-rate: 120–150 fps. Scale bar as shown therein.
Movie S5
Representative videos of the ex vivo beating heart in heart-specific Hand>CG9762-RNAi knockdown male Drosophila maintained on ALF standard cornmeal medium examined at 5 weeks age (top panel). Note the remarkable improvement of the RNAi genotype w.r.t. the 5 weeks (middle panel) and 3 weeks (bottom panel) old wildtype males included for reference. Movie duration: 15 seconds, Frame-rate: 120–150 fps. Scale bar as shown therein.