Supplementary Materials

A forward genetic screen identifies erythrocyte CD55 as essential for Plasmodium falciparum invasion

Elizabeth S. Egan, Rays H. Y. Jiang, Mischka A. Moechtar, Natasha S. Barteneva, Michael P. Weekes, Luis V. Nobre, Steven P. Gygi, Joao A. Paulo, Charles Frantzreb, Yoshihiko Tani, Junko Takahashi, Seishi Watanabe, Jonathan Goldberg, Aditya S. Paul, Carlo Brugnara, David E. Root, Roger C. Wiegand, John G. Doench, Manoj T. Duraisingh

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

Download Supplement
  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S10
  • Full Reference List
Data sets S1 to S5
Dataset S1. Gene list for erythrocyte proteome shRNA library. List of Gene symbols and Entrez Gene ID Numbers for genes included in the erythrocyte proteome shRNA library. Genes that were also included in the blood group secondary pool are indicated in bold type.

Dataset S2. RIGER analysis results for pooled shRNA screen of erythrocyte proteome. Erythropoiesis phenotypes associated with gene knockdown included a decrease in shRNA abundance on day 19 (decrease) or an increase in shRNA abundance on day 19 (increase) relative to the original library pool. RIGER analysis for each of 6 replicates was performed with weighted sum scores. Gene rank and Normalized Enrichment Scores (NES) are shown for each gene for each of 6 replicates.

Dataset S3. Interactome data. Pairwise interaction data and references are listed, in support of Figure S2.

Dataset S4. Phenotypes and expression patterns of erythropoiesis candidates. Data are presented for 116 hits. Expression data correspond to Fig. S3A. Gene Ontology (GO) annotation, gene description, and described mouse knockout phenotypes are listed.

Dataset S5. RIGER analysis for pooled shRNA screen of human blood group genes. RIGER analysis for each of 3 replicates was performed with weighted sum scores to identify candidate host factors for P. falciparum. Hairpin rank numbers, NES values gene rank numbers, and pvalues are shown for each gene.