Supplementary Materials

PI4P/phosphatidylserine countertransport at ORP5- and ORP8-mediated ER–plasma membrane contacts

Jeeyun Chung, Federico Torta, Kaori Masai, Louise Lucast, Heather Czapla, Lukas B. Tanner, Pradeep Narayanaswamy, Markus R. Wenk, Fubito Nakatsu, Pietro De Camilli

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S16
  • Table S1
  • Full Reference List

Images, Video, and Other Other Media

Movie S1
Dissociation of GFP-ORP5 from ER-PM contacts upon A1 treatment. Confocal live imaging of HeLa cells expressing GFP-ORP5 (green) and mRFP-PHPLCδ (red). 10-s intervals image acquisition. 100nM A1 was added at 30 sec. Scale bar, 10μm.
Movie S2
Dissociation of GFP-ORP8S from ER-PM contacts upon A1 treatment. Confocal live imaging of HeLa cells expressing GFP-ORP8S (green) and mRFP-PHPLCδ (red). 10-s intervals image acquisition. 100nM A1 was added at 30 sec. Scale bar, 10μm.
Movie S3
Dissociation of iRFP-P4M, but not mRFP-PHPLCδ , from ER-PM contacts upon A1 treatment. Confocal live imaging of HeLa cells expressing iRFP-P4M (cyan) and mRFPPHPLCδ (red). 10-s intervals image acquisition. 100nM A1 was added at 30 sec. Scale bar, 10μm.
Correction (5 October 2015): The number of phosphatidylserine molecules in the original fig. S16 did not reflect their higher concentration in the plasma membrane relative to the ER membrane. The figure has been corrected here. In addition, legends for the supplementary movies have been added.
The original version is accessible here.