Supplementary Materials

Oscillatory stress stimulation uncovers an Achilles' heel of the yeast MAPK signaling network

Amir Mitchell, Ping Wei, Wendell A. Lim

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S5
  • Tables S1 and S2
  • Full Reference List

Images, Video, and Other Other Media

Movie S1
Cell growth and osmoreponse under time-variant osmostress in a wild-type strain. A representative time-lapse microscopy movie depicting the cell growth and the osmotic response under different osmostress profiles (no stress, single step osmo-stress, and osmotic oscillations). The movie shows an overlay of images acquired from the DIC and yECitrine channels (images acquired every 15min). Stress starts one hour after the experiment started.
Movie S2
Cell growth and osmoreponse of wild-type and OspF strains. A representative time-lapse microscopy movie depicting the cell growth and the osmotic response under osmotic oscillations (period 16min) in the wild-type and feedback engineered strains. The movie shows an overlay of images acquired from the DIC and yECitrine channels (images acquired every 15min). Stress starts one hour after the experiment started.