Supplementary Materials

Structure of the Sec61 channel opened by a signal sequence

Rebecca M. Voorhees and Ramanujan S. Hegde

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S8
  • Tables S1 and S2
  • References
  • Movie legends S1 and S2

Images, Video, and Other Other Media

Movie S1
Movie S1 Morph between the ribosome-primed idle Sec61 complex (3J7Q) and the engaged Sec61 complex. The view is from the plan of the membrane looking toward the lateral gate; the ribosome would be on the bottom and ER lumen on the top. Only the transmembrane segments and the ribosome-interacting cytosolic loops are shown for clarity. Sec61α, β, and γ are in red, light blue, and tan, respectively. The signal sequence (cyan) appears in the engaged state at its position in the lateral gate.
Movie S2
Movie S2Morph between the ribosome-primed idle Sec61 complex (3J7Q) and the engaged Sec61 complex as viewed from the ER lumen. The lateral gate is on the bottom. Only the transmembrane segments are depicted for clarity. At the beginning of the animation, Sec61α, β, and γ are in red, light blue, and tan, respectively. The helices that rotate as a rigid body turn grey just before their movement. The signal sequence (cyan) appears in the engaged state at the end of the animation.