Supplementary Materials

Epigenetic (re)programming of caste-specific behavior in the ant Camponotus floridanus

Daniel F. Simola, Riley J. Graham, Cristina M. Brady, Brittany L. Enzmann, Claude Desplan, Anandasankar Ray, Laurence J. Zwiebel, Roberto Bonasio, Danny Reinberg, Jürgen Liebig, Shelley L. Berger

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S12
  • Tables S1 to S6
  • Captions for tables S7 to S10
  • Captions for movies S1 and S2
  • References
Table S7
Behavioral data for all trials performed following the starvation induced foraging assay. This table is available on Science Online as a Microsoft Excel file.
Table S8
Behavioral data for all trials performed using callow ants following the brain injection piggyback foraging assay.This table is available on Science Online as a tab-delimited text file.
Table S9
Single nucleotide polymorphisms (SNPs) in the C. floridanus genome.These 396,951 SNPs were utilized during mapping of high throughput sequencing data. This table is available on Science Online as a tab-delimited text file.
Table S10
Gene annotations used for all genomics analysis (OGS v3.6.2).This table is available on Science Online as a tab-delimited text file.

Images, Video, and Other Other Media

Movie S1
Starvation induced foraging assay.Three groups of 15 age-matched minors were isolated from a queen-right nest and placed in an isolation nest with water. After 24h, each nest was connected to a foraging arena containing 20% sugar water as bait and video was recorded for 70 min. In the movie, the cohort in the nest on the right exhibits foraging, while the left and middle nests do not. The lead scout begins to feed at 5s in the movie. Video shown at 2x speed. This movie is available on Science Online.
Movie S2
Brain injection procedure.A Minutin steel pin is used to puncture the cuticle at the ventral midline between the frontal carinae, slightly posterior to the eyes. A borosilicate glass needle preloaded with 1μL of liquid cargo is inserted into the punctured head cavity. Cargo is delivered via Eppendorf PatchMan and InjectMan NI 2 microinjection apparatus. In the movie, nonconjugated Cy3 fluorophore dissolved in 1μL water is used for visualization of cargo delivery. Video is shown at 2x speed. This movie is available on Science Online.