Supplementary Materials

Cyclic programmed cell death stimulates hormone signaling and root development in Arabidopsis

Wei Xuan, Leah R. Band, Robert P. Kumpf, Daniël Van Damme, Boris Parizot, Gieljan De Rop, Davy Opdenacker, Barbara K. Möller, Noemi Skorzinski, Maria F. Njo, Bert De Rybel, Dominique Audenaert, Moritz K. Nowack, Steffen Vanneste, Tom Beeckman

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S12
  • Table S1
  • Full Reference List

Images, Video, and Other Other Media

Movie S1
Movie of DR5rev:VENUS-N7 expression in a 3-day-old seedling for 14 hours. Red arrows indicate the disappearance of DR5 expression in the root cap; yellow arrows indicate the formation of a lateral root primordium. Movie is at 30 frames per second. Scale bar, 0.2 mm.
Movie S2
Movie of DR5rev:VENUS-N7 expression in a 3-day-old seedling after a gravistimulation (135° turn) for 20 hours. Red arrows indicate the disappearance of DR5 expression in the root cap during root bending; yellow arrows indicate a lateral root primordium. Movie is at 30 frames per second. Scale bar, 0.2 mm.
Movie S3
Movie of pPASPA3:NLS-tdTOMATO expression in a 3-day-old pPASPA3::NLS-tdTOMATO x DR5rev:VENUS-N7 seedling under normal condition over 18 hours. At the end of the movie, the corresponding DR5 expression (green) at 19 hours was included to visualize the position of lateral root primordia. Red arrows indicate the sites where PASPA3 stripes disappeared from the most distal root cap cell and green arrows indicated the positions of DR5 expressing lateral root primordia. Movie is at 30 frames per second. Scale bar, 0.2 mm.
Movie S4
Movie of pPASPA3:NLS-tdTOMATO expression in a 3-day-old pPASPA3::NLS-tdTOMATO x DR5rev:VENUS-N7 seedling after gravistimulation (135° turn) for 18 hours. At the end of the movie, the corresponding DR5 expression (green) at 19 hours was included to visualize the position of lateral root primordia. Red arrows indicate the sites where PASPA3 stripes disappeared from the most distal root cap cell and green arrows indicated the positions of DR5 expressing lateral root primordia. Movie is at 30 frames per second. Scale bar, 0.2 mm.
Movie S5
Movie of DR5:Luciferase expression in 3-day-old seedlings Col-0 and 35S:SMB-GR Dex-treated seedling over 24 hours. One root from the Col-0 seedlings (on the left) and five roots from the 35S:SMB-GR seedlings were imaged over time. Movie is at 24 frames per second. Scale bar, 0.5 cm.
Movie S6
Movie of D2R2 expression in a 3-day-old seedling root tip over 170 minutes. Seedling was placed in the growth chamber covered with 1/2 MS agar, and horizontally scanned by confocal every 10 min. 3D-projection of the images were performed in ImageJ and stacked as video files. Yellow box indicates the region where the disappearance of YFP signals in epidermal cells and the PCD of lateral root cap cells were observed. Movie is at 6 frames per second. Scale bar, 50 μm.
Movie S7
Movie of predicted dynamics of deposited auxin over 300 seconds after programmed cell death of the final lateral root cap cells in wild type. Panels show dynamics for wild type, defect in IBA-IAA conversion, defect in AUX1-mediated influx and defect in carrier-mediated efflux. Movie represents 20 frames per second where each frame represents 1 second. Scale bar, 50 μm.
Movie S8
Movie of DR5rev:VENUS-N7 expression in 3-day-old Col-0 seedlings treated with Mock, 10μM NPA, 0.3μM BUM, and 3 μM BZ-IAA for 18 hours. Movie is at 30 frames per second. Scale bar, 0.5 mm.
Movie S9
Movie of DR5:Luciferase expression in a 3-day-old Col-0 seedling treated with Mock, 10μM NPA, 0.3μM BUM, and 3 μM BZ-IAA for 20 hours. Movie is at 24 frames per second. Scale bar, 0.1 cm.