Supplementary Materials

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S5
  • Full Reference List
  • Captions for Movies S1 to S6

Images, Video, and Other Media

Movie S1
Microsphere bead flow and CSF dynamics within the ptk7/+ rhombencephalic ventricle. 50 frames = 10 seconds.
Movie S2
Microsphere bead flow and CSF dynamics within the ptk7 mutant rhombencephalic ventricle. 50 frames = 10 seconds.
Movie S3
foxj1a enhancer element drives gene expression within the midline of the brain and spinal cord. Three-dimensonal reconstruction of a z-series through the trunk and neural tube of a 48hpf Tg(foxj1a::eGFP) embryo revealing eGFP fluorescence in the dorsal roof plate and ventral floor plate.
Movie S4
Microsphere bead flow and CSF dynamics within ptk7 + Tg(foxj1a::ptk7) zebrafish rhombencephalic ventricle. 50 frames = 10 seconds.
Movie S5
Microsphere bead flow and CSF dynamics within the c21orƒ59TS mutant rhombencephalic ventricle. Live imaging movie showing flow of fluorescent microspheres in 10 seconds. Tissue was maintained at 30°C for the duration of the dissection and imaging
Movie S6
Rescued c21orƒ59TS mutant zebrafish model idiopathic scoliosis. Threedimensional microCT reconstruction (18 μm isotrophic resolution) of an adult c21orƒ59TS mutant zebrafish, which was raised through the first 5 days of embryonic development at permissive temperatures (25°C) before being shifted to a restrictive temperature of 30°C.