Supplementary Materials

Cilia-based flow network in the brain ventricles

Regina Faubel, Christian Westendorf, Eberhard Bodenschatz, Gregor Eichele

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S6
  • Table S1
  • Full Reference List
  • Captions for Movies S1 to S11

Images, Video, and Other Media

Movie S1
Characteristic transport pathways of FITC-dextran tracer delivered at the entry of the v3V. When applied at flow 1 or 2 (Fig. 1C), the tracer first formed a narrow stream that subsequently was dispersed in a fan-like fashion and subsequently detached from the near-wall flow (sequences 1 and 2). Injections at the head of flow 3 gave rise to a forward-migrating “slug-like� body that first progressed ventrally, made a sharp turn towards posterior, and then turned again to be targeted ventrally (sequence 3).
Movie S2
The separatrix in a v3V whole mount. In a whole mount of the v3V the dorsal seam was slightly opened and particles were delivered into flow 4. In this dorsal aspect particles first streamed along the roof of the v3V but at the separatrix (S) abruptly plunged mirror-symmetrically into the depth of the v3V.
Moviei S3
Analysis of cilia movement. For details, see Materials and Methods.
Movie S4
Slow motion movie of beating cilia at the separatrix. Cilia bundles on the left and right beat in oblique but opposite directions (arrows 3 and 4).
Movie S5
Slow motion movie of beating cilia at the entrance of the v3V. Cilia bundles predominantly beat in the three directions creating flows 1, 2, and 3 of Fig. 1C.
Movie S6
Slow motion movie of cilia beating underneath the separatrix at ZT23. A diagram (left) highlights the salient features of the cilia movie (right) including the position of the separatrix (dashes) and of the whirl (W). Note cilia beat in a counter-clockwise manner.
Movie S7
Slow motion movie of cilia beating underneath the separatrix at ZT10. A diagram (left) shows the separatrix (dashes) and the transition between flows 4 and 5.
Movie S8
Progression of FITC-dextran at ZT10 defines a dorsoventral boundary. Two sequential low-dose injections (1 and 2) of FITC-dextran into flow 4 progressed in a dorsoventral direction. Trajectories of 1 and 2 are outlined in Fig. 3C.
Movie S9
Progression of a high dose of FITC-dextran at ZT10 defines a dorsoventral boundary. FITC-dextran injected into flow 4 flowed in a dorsoventral direction (see also Fig. 3C).
Movie S10
Progression of FITC-dextran at ZT23 defines a horizontal boundary. Three sequential low-dose injections (1-3) flowed towards the whirl where they exited near-wall flow. Note the reference mark created by injection of FITC-dextran into the ependyma (see also Fig. 3D).
Movie S11
Progression of high doses of FITC-dextran at ZT23 defines a horizontal boundary. FITC-dextran injected into flow 4 first progressed towards the separatrix where it was lifted up by the whirl thus departing from near-wall flow. After around 13 sec, FITC-dextran progressed anteriorly and remained in the dorsal part of the ventricle. This flow was no longer near-wall flow (see also Fig. 3D).