Supplementary Materials

Systems-level analysis of mechanisms regulating yeast metabolic flux

Sean R. Hackett, Vito R. T. Zanotelli, Wenxin Xu, Jonathan Goya, Junyoung O. Park, David H. Perlman, Patrick A. Gibney, David Botstein, John D. Storey, Joshua D. Rabinowitz

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Figs. S1 to S15
  • Tables S1 to S6
  • Captions for Tables S7 to S9
  • References
Table S7
Summary of 729 literature-reported activators and inhibitors (across all organisms) of the 56 studied reactions. The R2 between measured flux and the regulators kinetic model is listed. The AICc of each regulator is listed if this regulator significantly improves upon the fit based on 1-by-1 testing without Bayesian analysis (q < 0.1). Note that higher R2 and lower AICc indicate a better fit.
Table S8
Metabolic leverage for each of the 28 studied metabolic reactions that was adequately fit (R2 > 0.35) with the best-supported reaction equation either involving no regulation or biochemically validated regulation. Best estimate reflects the metabolic leverage determined using the best estimate of each reactions kinetic parameters. To determine the robustness of the metabolic leverage measurements, these were repeated using 2000 different well-fitting kinetic parameter sets. The interquartile range (IQR) and 95% credibility interval of these samples is reported.:
Table S9
Experimentally measured metabolite and protein abundances and boundary fluxes.