Supplementary Materials

Assembly of a nucleus-like structure during viral replication in bacteria

Vorrapon Chaikeeratisak, Katrina Nguyen, Kanika Khanna, Axel F. Brilot, Marcella L. Erb, Joanna K. C. Coker, Anastasia Vavilina, Gerald L. Newton, Robert Buschauer, Kit Pogliano, Elizabeth Villa, David A. Agard, Joe Pogliano

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S11
  • Tables S1 to S3
  • Captions for Movies S1 to S8
  • References (1320)

Images, Video, and Other Media

Movie S1
Time-lapse imaging, images taken every minute for 90 minutes, of an infected cell containing GFP-gp105 (green) and mCherry-PhuZ (red) beginning at 30 minutes post infection (mpi). The gp105 shell grows as infection progresses.
Movie S2
Time-lapse imaging, images taken every minute for 30 minutes, of an infected cell containing GFP-gp105 (green) and mCherry-PhuZ (red) beginning at 60 mpi. The gp105 shell oscillates back and forth at the midcell, suggesting that the PhuZ spindle exerts pushing forces.
Movie S3
Time-lapse imaging, images taken every 2 seconds for 1 minute, of an infected cell containing GFP-gp105 (green) and mCherry-PhuZ (red) beginning at 60 mpi. The gp105 shell rotates.
Movie S4
Time-lapse imaging, images taken every minute for 30 minutes, of an infected cell containing gp237-GFP (green) beginning at 20 mpi. gp237-GFP (RecA-like protein) translocates into the phage compartment.
Movie S5
Time-lapse imaging, images taken every 2 seconds for 2 minutes, of an infected cell containing gp237-GFP (green) and mCherry-gp105 (red) beginning at 70 mpi. gp237-GFP (RecA-like protein), inside the gp105 shell, forms foci and rotates along with the phage compartment
Movie S6
Time-lapse imaging, images taken every minute for 19 minutes, of an infected cell containing gp200-GFP (green) and mCherry-gp105 (red) beginning at 40 mpi. The gp200 capsids move through the cell within 1 minute to accumulate on the gp105 shell
Movie S7
Time-lapse imaging, images taken every 5 seconds for 10 minutes, of an infected cell containing gp200-GFP (green) and mCherry-gp105 (red) beginning at 48 mpi. The gp200 capsids are docked on the gp105 shell as it rotates.
Movie S8
Movie of cryo-electron tomography of the phage compartment during 201φ2-1 infection in P. chlororaphis. Segmentation of the tomogram shows extracted structures, including the shell (blue), capsids (green), the cytoplasmic membrane (pink), the outer membrane (red), phage tails (cyan) and the ribosomes (yellow).