Supplementary Materials

T cell costimulatory receptor CD28 is a primary target for PD-1–mediated inhibition

Enfu Hui, Jeanne Cheung, Jing Zhu, Xiaolei Su, Marcus J. Taylor, Heidi A. Wallweber, Dibyendu K. Sasmal, Jun Huang, Jeong M. Kim, Ira Mellman, Ronald D. Vale

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S15
  • Tables S1 to S3
  • Captions for Movies S1 and S2
  • References

Images, Video, and Other Media

Movie S1
A TIRF microscopy time-lapse video of the representative OT-I cell co-expressing mouse PD-1–mCherry, mouse CD28–mGFP after bilayer contact, as shown in Fig. 3A. PD-1, CD28 and TCR were rendered in red, green and cyan, respectively, using Fiji. Interval: 5 sec.
Movie S2
A TIRF microscopy time-lapse video of the representative OT-I cell co-expressing mouse PD-1–mCherry, mouse CD28–mGFP after contacting a bilayer that contains pMHC, LFA and B7.1, but lacks PD-L1, as shown in fig. S13. PD-1, CD28 and TCR were rendered in red, green and cyan, respectively, using Fiji. Interval: 5 sec.